Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Ctcf

Cell type

Cell type Class
Blood
Cell type
Bone Marrow Cells
MeSH Description
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.

Attributes by original data submitter

Sample

source_name
ERY cells
cell type
bone marrow derived progenitor cells
facs cell isolation sort markers
CD4-, CD8-, IL7Ra-, CD11b-, Ly6g-, CD45R-, Ter119+, CD44+, FSCLow
strain
C57BL/6
chip antibody
CTCF (Millipore 07-729)
average chromatin size as measured between a 100-500 bp window using the agilent bioanalyzer 7500 chip
276 bp

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were prepared from sonicated nuclei and protein-DNA complexes were isolated with antibody. ChIP-seq samples were prepared by end repair, 3' adenylation, and adaptor ligation using Illumina's TruSeq ChIP-seq Sample Preparation Kit.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
33164768
Reads aligned (%)
90.2
Duplicates removed (%)
50.2
Number of peaks
1552 (qval < 1E-05)

mm9

Number of total reads
33164768
Reads aligned (%)
90.0
Duplicates removed (%)
50.3
Number of peaks
1648 (qval < 1E-05)

Base call quality data from DBCLS SRA