Antigen

Antigen Class

TFs and others

Antigen

Ctcf

Cell type

Cell type Class

Blood

Cell type

Bone Marrow Cells

MeSH Description

Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.

Sample

source_name

ERY cells

cell type

bone marrow derived progenitor cells

facs cell isolation sort markers

CD4-, CD8-, IL7Ra-, CD11b-, Ly6g-, CD45R-, Ter119+, CD44+, FSCLow

strain

C57BL/6

chip antibody

CTCF (Millipore 07-729)

average chromatin size as measured between a 100-500 bp window using the agilent bioanalyzer 7500 chip

276 bp

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were prepared from sonicated nuclei and protein-DNA complexes were isolated with antibody. ChIP-seq samples were prepared by end repair, 3' adenylation, and adaptor ligation using Illumina's TruSeq ChIP-seq Sample Preparation Kit.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

33164768

Reads aligned (%)

90.2

Duplicates removed (%)

50.2

Number of peaks

1552 (qval < 1E-05)

mm9

Number of total reads

33164768

Reads aligned (%)

90.0

Duplicates removed (%)

50.3

Number of peaks

1648 (qval < 1E-05)